Making its debut in 1965, the original Gatorland Iron Horse gave visitors a glimpse of the south end of the park as it carried passengers over alligators and by the animal displays on its journey back to the station. After 35 years of loyal service carrying millions of passengers, the old Iron Horse was finally retired in 2000 and a new station was built.

Making its inaugural run on July 1, 2001, the new Gatorland Express departed from its brand new train station carrying passengers around the Jungle Crocs and Breeding Marsh/Bird Rookery areas. The train makes a stop at the very South end of the park close to Pearl's Smokehouse and the Alligator Wrestling Stadium. Visitors can still see the old original Gatorland Iron Horse on display just outside the new train station.

Also located at the Gatorland Train Station is Jorge's Fun Face Painting for both kids and adults. From swirling glitter designs to Spiderman faces, kids of all ages will enjoy this fun and creative experience.